Purification:Article Title: Endogenous IFITMs boost SARS-coronavirus 1 and 2 replication whereas overexpression inhibits infection by relocalizing ACE2.
Article Snippet: .. Antibodies IRDye 680RD Goat anti-Rabbit IgG (H + L) LI-COR Cat926-68071 IRDye 800CW Goat anti-Mouse IgG (H + L) LI-COR Cat# 926-32210 IRDye 800RD Goat anti-Rat IgG (H + L) LI-COR Cat# 925-32219 HCoV-229E Nucleocapsid Antibody SinoBiological Cat# 40640-T62 Anti-Coronavirus NL63 Ingenasa M.30.HCo.I2D4 Anti-Coronavirus Antibody, OC-43 strain Millipore Cat# MAB9012 Purified anti-GAPDH Antibody Biolegend Cat# 607902 Rabbit polyclonal anti-IFITM1 Cell signaling Cat# 13126 S Rabbit polyclonal anti-IFITM2 Cell signaling Cat# 13530S Rabbit polyclonal anti-IFITM3 Cell signaling Cat# 59212S V5-Tag (E9H8O) Mouse mAb Cell signaling Cat# 80076 SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody SinoBiological Cat# 40588-MM124 Rabbit polyclonal anti-ACE2 Abcam Cat# ab15348 Mouse monoclonal anti-flag Sigma-Aldrich Cat# F1804 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 Rabbit monoclonal Alexa Fluor 647 anti-CD46 Abcam Cat# 275208 Rat Monoclonal PerCP-Cy5.5 anti-CD4 Biolegend Cat# 317428 Bacterial and virus strains VSVDG(GFP)*VSV-G Klaus Conzelmann N/A hCoV-229E ATCC Cat# VR-740TM hCoV-NL63 Lia van der Hoek N/A hCoV-OC43 ATCC Cat# CR-1558TM Renilla hCoV-229E Volker Thiel N/A BetaCoV/Netherlands/01/NL/2020 European Virus Archive Cat# 010V 03903 VSVDG(GFP)*VSV-G Klaus Conzelmann N/A BetaCoV/Netherlands/01/NL/2020 European Virus Archive Cat# 010V 03903 BetaCoV/B.1.617.2/Delta Florian Schmidt (University of Bonn) N/A BetaCoV/Munich/BavPat1/2020 GISAID; Wölfel et al., 202047 EPI_ISL_406862 SARS-CoV (rSCV, Frankfurt-1 virus isolate) Pfefferle et al., 200929 Genbank accession no. AY310120 rMERS-CoV, MERS-CoV/EMC2012 Muth et al., 201748 Genbank accession no. NC_019843.3 Chemicals, peptides, and recombinant proteins L-Glutamine Pan Biotech Cat# P04-80100 DAPI Sigma-Aldrich Cat# D9542-1MG; CAS: 28718-90-3 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat# 41965039 Dulbecco’s Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 14190094 Minimum Essential Medium Eagle (MEM) Sigma-Aldrich Cat# M4655 Opti-MEM Gibco Cat# M3942 Roswell Park Memorial Institute (RPMI) Gibco Cat# 22400089 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 non-essential amino acids Sigma-Aldrich Cat# M7145 (Continued on next page) iScience 26, 106395, April 21, 2023 17 .. Sodium pyruvate Sigma-Aldrich Cat# S8636 FCS Gibco Cat# 10270106 Trypsin EDTA Gibco Cat# T3924 Versene Thermo Fisher Cat# 15040066 TMB peroxidase substrate (Sure blue) Medac Cat# 52-00-04 BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Lipofectamine RNAiMAX ThermoFisher Cat# 13778075 SuperScript III Platinum One-Step qRT-PCR Kit Invitrogen Cat# 11732088 Renilla Luciferase Assay kit Promega Cat# E2810 Quick RNA Miniprep Kit ZYMO research Cat# R1055 QIAamp Viral RNA Kits QIAGEN Cat# 52906 Luciferase Assay Kit Promega Cat# E1500 Mowiol 4-88 ROTH Cat# 0713.1 Blocker Casein in PBS Thermo Fisher Cat# 37528 TaqMan Fast Virus 1-Step Master Mix ThermoFisher Cat# 4444436 Tween 20 Sigma-Aldrich Cat# P7949500ML Triton X-100 Sigma-Aldrich Cat#T9284 100ML GAPD (GAPDH) Endogenous Control ThermoFisher Cat# 4352338E b-Mercaptoethanol Sigma-Aldrich Cat# 444203 Immobilon-FL PVDF membranes Merck Millipore Cat# IPVH00010 Paraformaldehyde Merck, Darmstadt Cat# 104005 TransIT-LT1 Mirus Cat# MIR2300 NuPAGE 4G12% Bis-Tris Gels ThermoFisher Cat# NP0321BOX Synthetic SARS-CoV-2-RNA Twist Bioscience Cat# 102019 RNAiMAX ThermoFisher Cat# LMRNA015 Recombinant IFN-a2 pbl assay science Cat# 11101-1 Recombinant IFN-b R&D systems Cat# 8499-IF-010 Recombinant IFN-g Sigma-Aldrich Cat# I3265 Recombinant IFN- l3 CHO Cat# 5259-IL-025 Cytochalasin D ThermoFisher Cat# PHZ1063 Agarose Sigma-Aldrich Cat# A9539 Experimental models: Cell lines Human: Calu-3 ATCC or M. Frick Cat# HTB-55 or N/A Human: Caco2 Holger Barth Human: Huh7.5 Ralf Bartenschlager Human: Huh7 Anna-Laura Kretz LLCMK2 by Lia van der Hoek
Virus:Article Title: Endogenous IFITMs boost SARS-coronavirus 1 and 2 replication whereas overexpression inhibits infection by relocalizing ACE2.
Article Snippet: .. Antibodies IRDye 680RD Goat anti-Rabbit IgG (H + L) LI-COR Cat926-68071 IRDye 800CW Goat anti-Mouse IgG (H + L) LI-COR Cat# 926-32210 IRDye 800RD Goat anti-Rat IgG (H + L) LI-COR Cat# 925-32219 HCoV-229E Nucleocapsid Antibody SinoBiological Cat# 40640-T62 Anti-Coronavirus NL63 Ingenasa M.30.HCo.I2D4 Anti-Coronavirus Antibody, OC-43 strain Millipore Cat# MAB9012 Purified anti-GAPDH Antibody Biolegend Cat# 607902 Rabbit polyclonal anti-IFITM1 Cell signaling Cat# 13126 S Rabbit polyclonal anti-IFITM2 Cell signaling Cat# 13530S Rabbit polyclonal anti-IFITM3 Cell signaling Cat# 59212S V5-Tag (E9H8O) Mouse mAb Cell signaling Cat# 80076 SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody SinoBiological Cat# 40588-MM124 Rabbit polyclonal anti-ACE2 Abcam Cat# ab15348 Mouse monoclonal anti-flag Sigma-Aldrich Cat# F1804 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 Rabbit monoclonal Alexa Fluor 647 anti-CD46 Abcam Cat# 275208 Rat Monoclonal PerCP-Cy5.5 anti-CD4 Biolegend Cat# 317428 Bacterial and virus strains VSVDG(GFP)*VSV-G Klaus Conzelmann N/A hCoV-229E ATCC Cat# VR-740TM hCoV-NL63 Lia van der Hoek N/A hCoV-OC43 ATCC Cat# CR-1558TM Renilla hCoV-229E Volker Thiel N/A BetaCoV/Netherlands/01/NL/2020 European Virus Archive Cat# 010V 03903 VSVDG(GFP)*VSV-G Klaus Conzelmann N/A BetaCoV/Netherlands/01/NL/2020 European Virus Archive Cat# 010V 03903 BetaCoV/B.1.617.2/Delta Florian Schmidt (University of Bonn) N/A BetaCoV/Munich/BavPat1/2020 GISAID; Wölfel et al., 202047 EPI_ISL_406862 SARS-CoV (rSCV, Frankfurt-1 virus isolate) Pfefferle et al., 200929 Genbank accession no. AY310120 rMERS-CoV, MERS-CoV/EMC2012 Muth et al., 201748 Genbank accession no. NC_019843.3 Chemicals, peptides, and recombinant proteins L-Glutamine Pan Biotech Cat# P04-80100 DAPI Sigma-Aldrich Cat# D9542-1MG; CAS: 28718-90-3 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat# 41965039 Dulbecco’s Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 14190094 Minimum Essential Medium Eagle (MEM) Sigma-Aldrich Cat# M4655 Opti-MEM Gibco Cat# M3942 Roswell Park Memorial Institute (RPMI) Gibco Cat# 22400089 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 non-essential amino acids Sigma-Aldrich Cat# M7145 (Continued on next page) iScience 26, 106395, April 21, 2023 17 .. Sodium pyruvate Sigma-Aldrich Cat# S8636 FCS Gibco Cat# 10270106 Trypsin EDTA Gibco Cat# T3924 Versene Thermo Fisher Cat# 15040066 TMB peroxidase substrate (Sure blue) Medac Cat# 52-00-04 BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Lipofectamine RNAiMAX ThermoFisher Cat# 13778075 SuperScript III Platinum One-Step qRT-PCR Kit Invitrogen Cat# 11732088 Renilla Luciferase Assay kit Promega Cat# E2810 Quick RNA Miniprep Kit ZYMO research Cat# R1055 QIAamp Viral RNA Kits QIAGEN Cat# 52906 Luciferase Assay Kit Promega Cat# E1500 Mowiol 4-88 ROTH Cat# 0713.1 Blocker Casein in PBS Thermo Fisher Cat# 37528 TaqMan Fast Virus 1-Step Master Mix ThermoFisher Cat# 4444436 Tween 20 Sigma-Aldrich Cat# P7949500ML Triton X-100 Sigma-Aldrich Cat#T9284 100ML GAPD (GAPDH) Endogenous Control ThermoFisher Cat# 4352338E b-Mercaptoethanol Sigma-Aldrich Cat# 444203 Immobilon-FL PVDF membranes Merck Millipore Cat# IPVH00010 Paraformaldehyde Merck, Darmstadt Cat# 104005 TransIT-LT1 Mirus Cat# MIR2300 NuPAGE 4G12% Bis-Tris Gels ThermoFisher Cat# NP0321BOX Synthetic SARS-CoV-2-RNA Twist Bioscience Cat# 102019 RNAiMAX ThermoFisher Cat# LMRNA015 Recombinant IFN-a2 pbl assay science Cat# 11101-1 Recombinant IFN-b R&D systems Cat# 8499-IF-010 Recombinant IFN-g Sigma-Aldrich Cat# I3265 Recombinant IFN- l3 CHO Cat# 5259-IL-025 Cytochalasin D ThermoFisher Cat# PHZ1063 Agarose Sigma-Aldrich Cat# A9539 Experimental models: Cell lines Human: Calu-3 ATCC or M. Frick Cat# HTB-55 or N/A Human: Caco2 Holger Barth Human: Huh7.5 Ralf Bartenschlager Human: Huh7 Anna-Laura Kretz LLCMK2 by Lia van der Hoek
Recombinant:Article Title: Endogenous IFITMs boost SARS-coronavirus 1 and 2 replication whereas overexpression inhibits infection by relocalizing ACE2.
Article Snippet: .. Antibodies IRDye 680RD Goat anti-Rabbit IgG (H + L) LI-COR Cat926-68071 IRDye 800CW Goat anti-Mouse IgG (H + L) LI-COR Cat# 926-32210 IRDye 800RD Goat anti-Rat IgG (H + L) LI-COR Cat# 925-32219 HCoV-229E Nucleocapsid Antibody SinoBiological Cat# 40640-T62 Anti-Coronavirus NL63 Ingenasa M.30.HCo.I2D4 Anti-Coronavirus Antibody, OC-43 strain Millipore Cat# MAB9012 Purified anti-GAPDH Antibody Biolegend Cat# 607902 Rabbit polyclonal anti-IFITM1 Cell signaling Cat# 13126 S Rabbit polyclonal anti-IFITM2 Cell signaling Cat# 13530S Rabbit polyclonal anti-IFITM3 Cell signaling Cat# 59212S V5-Tag (E9H8O) Mouse mAb Cell signaling Cat# 80076 SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody SinoBiological Cat# 40588-MM124 Rabbit polyclonal anti-ACE2 Abcam Cat# ab15348 Mouse monoclonal anti-flag Sigma-Aldrich Cat# F1804 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 Rabbit monoclonal Alexa Fluor 647 anti-CD46 Abcam Cat# 275208 Rat Monoclonal PerCP-Cy5.5 anti-CD4 Biolegend Cat# 317428 Bacterial and virus strains VSVDG(GFP)*VSV-G Klaus Conzelmann N/A hCoV-229E ATCC Cat# VR-740TM hCoV-NL63 Lia van der Hoek N/A hCoV-OC43 ATCC Cat# CR-1558TM Renilla hCoV-229E Volker Thiel N/A BetaCoV/Netherlands/01/NL/2020 European Virus Archive Cat# 010V 03903 VSVDG(GFP)*VSV-G Klaus Conzelmann N/A BetaCoV/Netherlands/01/NL/2020 European Virus Archive Cat# 010V 03903 BetaCoV/B.1.617.2/Delta Florian Schmidt (University of Bonn) N/A BetaCoV/Munich/BavPat1/2020 GISAID; Wölfel et al., 202047 EPI_ISL_406862 SARS-CoV (rSCV, Frankfurt-1 virus isolate) Pfefferle et al., 200929 Genbank accession no. AY310120 rMERS-CoV, MERS-CoV/EMC2012 Muth et al., 201748 Genbank accession no. NC_019843.3 Chemicals, peptides, and recombinant proteins L-Glutamine Pan Biotech Cat# P04-80100 DAPI Sigma-Aldrich Cat# D9542-1MG; CAS: 28718-90-3 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat# 41965039 Dulbecco’s Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 14190094 Minimum Essential Medium Eagle (MEM) Sigma-Aldrich Cat# M4655 Opti-MEM Gibco Cat# M3942 Roswell Park Memorial Institute (RPMI) Gibco Cat# 22400089 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 non-essential amino acids Sigma-Aldrich Cat# M7145 (Continued on next page) iScience 26, 106395, April 21, 2023 17 .. Sodium pyruvate Sigma-Aldrich Cat# S8636 FCS Gibco Cat# 10270106 Trypsin EDTA Gibco Cat# T3924 Versene Thermo Fisher Cat# 15040066 TMB peroxidase substrate (Sure blue) Medac Cat# 52-00-04 BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Lipofectamine RNAiMAX ThermoFisher Cat# 13778075 SuperScript III Platinum One-Step qRT-PCR Kit Invitrogen Cat# 11732088 Renilla Luciferase Assay kit Promega Cat# E2810 Quick RNA Miniprep Kit ZYMO research Cat# R1055 QIAamp Viral RNA Kits QIAGEN Cat# 52906 Luciferase Assay Kit Promega Cat# E1500 Mowiol 4-88 ROTH Cat# 0713.1 Blocker Casein in PBS Thermo Fisher Cat# 37528 TaqMan Fast Virus 1-Step Master Mix ThermoFisher Cat# 4444436 Tween 20 Sigma-Aldrich Cat# P7949500ML Triton X-100 Sigma-Aldrich Cat#T9284 100ML GAPD (GAPDH) Endogenous Control ThermoFisher Cat# 4352338E b-Mercaptoethanol Sigma-Aldrich Cat# 444203 Immobilon-FL PVDF membranes Merck Millipore Cat# IPVH00010 Paraformaldehyde Merck, Darmstadt Cat# 104005 TransIT-LT1 Mirus Cat# MIR2300 NuPAGE 4G12% Bis-Tris Gels ThermoFisher Cat# NP0321BOX Synthetic SARS-CoV-2-RNA Twist Bioscience Cat# 102019 RNAiMAX ThermoFisher Cat# LMRNA015 Recombinant IFN-a2 pbl assay science Cat# 11101-1 Recombinant IFN-b R&D systems Cat# 8499-IF-010 Recombinant IFN-g Sigma-Aldrich Cat# I3265 Recombinant IFN- l3 CHO Cat# 5259-IL-025 Cytochalasin D ThermoFisher Cat# PHZ1063 Agarose Sigma-Aldrich Cat# A9539 Experimental models: Cell lines Human: Calu-3 ATCC or M. Frick Cat# HTB-55 or N/A Human: Caco2 Holger Barth Human: Huh7.5 Ralf Bartenschlager Human: Huh7 Anna-Laura Kretz LLCMK2 by Lia van der Hoek
Modification:Article Title: Endogenous IFITMs boost SARS-coronavirus 1 and 2 replication whereas overexpression inhibits infection by relocalizing ACE2.
Article Snippet: .. Antibodies IRDye 680RD Goat anti-Rabbit IgG (H + L) LI-COR Cat926-68071 IRDye 800CW Goat anti-Mouse IgG (H + L) LI-COR Cat# 926-32210 IRDye 800RD Goat anti-Rat IgG (H + L) LI-COR Cat# 925-32219 HCoV-229E Nucleocapsid Antibody SinoBiological Cat# 40640-T62 Anti-Coronavirus NL63 Ingenasa M.30.HCo.I2D4 Anti-Coronavirus Antibody, OC-43 strain Millipore Cat# MAB9012 Purified anti-GAPDH Antibody Biolegend Cat# 607902 Rabbit polyclonal anti-IFITM1 Cell signaling Cat# 13126 S Rabbit polyclonal anti-IFITM2 Cell signaling Cat# 13530S Rabbit polyclonal anti-IFITM3 Cell signaling Cat# 59212S V5-Tag (E9H8O) Mouse mAb Cell signaling Cat# 80076 SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody SinoBiological Cat# 40588-MM124 Rabbit polyclonal anti-ACE2 Abcam Cat# ab15348 Mouse monoclonal anti-flag Sigma-Aldrich Cat# F1804 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 Rabbit monoclonal Alexa Fluor 647 anti-CD46 Abcam Cat# 275208 Rat Monoclonal PerCP-Cy5.5 anti-CD4 Biolegend Cat# 317428 Bacterial and virus strains VSVDG(GFP)*VSV-G Klaus Conzelmann N/A hCoV-229E ATCC Cat# VR-740TM hCoV-NL63 Lia van der Hoek N/A hCoV-OC43 ATCC Cat# CR-1558TM Renilla hCoV-229E Volker Thiel N/A BetaCoV/Netherlands/01/NL/2020 European Virus Archive Cat# 010V 03903 VSVDG(GFP)*VSV-G Klaus Conzelmann N/A BetaCoV/Netherlands/01/NL/2020 European Virus Archive Cat# 010V 03903 BetaCoV/B.1.617.2/Delta Florian Schmidt (University of Bonn) N/A BetaCoV/Munich/BavPat1/2020 GISAID; Wölfel et al., 202047 EPI_ISL_406862 SARS-CoV (rSCV, Frankfurt-1 virus isolate) Pfefferle et al., 200929 Genbank accession no. AY310120 rMERS-CoV, MERS-CoV/EMC2012 Muth et al., 201748 Genbank accession no. NC_019843.3 Chemicals, peptides, and recombinant proteins L-Glutamine Pan Biotech Cat# P04-80100 DAPI Sigma-Aldrich Cat# D9542-1MG; CAS: 28718-90-3 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat# 41965039 Dulbecco’s Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 14190094 Minimum Essential Medium Eagle (MEM) Sigma-Aldrich Cat# M4655 Opti-MEM Gibco Cat# M3942 Roswell Park Memorial Institute (RPMI) Gibco Cat# 22400089 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 non-essential amino acids Sigma-Aldrich Cat# M7145 (Continued on next page) iScience 26, 106395, April 21, 2023 17 .. Sodium pyruvate Sigma-Aldrich Cat# S8636 FCS Gibco Cat# 10270106 Trypsin EDTA Gibco Cat# T3924 Versene Thermo Fisher Cat# 15040066 TMB peroxidase substrate (Sure blue) Medac Cat# 52-00-04 BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Lipofectamine RNAiMAX ThermoFisher Cat# 13778075 SuperScript III Platinum One-Step qRT-PCR Kit Invitrogen Cat# 11732088 Renilla Luciferase Assay kit Promega Cat# E2810 Quick RNA Miniprep Kit ZYMO research Cat# R1055 QIAamp Viral RNA Kits QIAGEN Cat# 52906 Luciferase Assay Kit Promega Cat# E1500 Mowiol 4-88 ROTH Cat# 0713.1 Blocker Casein in PBS Thermo Fisher Cat# 37528 TaqMan Fast Virus 1-Step Master Mix ThermoFisher Cat# 4444436 Tween 20 Sigma-Aldrich Cat# P7949500ML Triton X-100 Sigma-Aldrich Cat#T9284 100ML GAPD (GAPDH) Endogenous Control ThermoFisher Cat# 4352338E b-Mercaptoethanol Sigma-Aldrich Cat# 444203 Immobilon-FL PVDF membranes Merck Millipore Cat# IPVH00010 Paraformaldehyde Merck, Darmstadt Cat# 104005 TransIT-LT1 Mirus Cat# MIR2300 NuPAGE 4G12% Bis-Tris Gels ThermoFisher Cat# NP0321BOX Synthetic SARS-CoV-2-RNA Twist Bioscience Cat# 102019 RNAiMAX ThermoFisher Cat# LMRNA015 Recombinant IFN-a2 pbl assay science Cat# 11101-1 Recombinant IFN-b R&D systems Cat# 8499-IF-010 Recombinant IFN-g Sigma-Aldrich Cat# I3265 Recombinant IFN- l3 CHO Cat# 5259-IL-025 Cytochalasin D ThermoFisher Cat# PHZ1063 Agarose Sigma-Aldrich Cat# A9539 Experimental models: Cell lines Human: Calu-3 ATCC or M. Frick Cat# HTB-55 or N/A Human: Caco2 Holger Barth Human: Huh7.5 Ralf Bartenschlager Human: Huh7 Anna-Laura Kretz LLCMK2 by Lia van der Hoek
Saline:Article Title: Endogenous IFITMs boost SARS-coronavirus 1 and 2 replication whereas overexpression inhibits infection by relocalizing ACE2.
Article Snippet: .. Antibodies IRDye 680RD Goat anti-Rabbit IgG (H + L) LI-COR Cat926-68071 IRDye 800CW Goat anti-Mouse IgG (H + L) LI-COR Cat# 926-32210 IRDye 800RD Goat anti-Rat IgG (H + L) LI-COR Cat# 925-32219 HCoV-229E Nucleocapsid Antibody SinoBiological Cat# 40640-T62 Anti-Coronavirus NL63 Ingenasa M.30.HCo.I2D4 Anti-Coronavirus Antibody, OC-43 strain Millipore Cat# MAB9012 Purified anti-GAPDH Antibody Biolegend Cat# 607902 Rabbit polyclonal anti-IFITM1 Cell signaling Cat# 13126 S Rabbit polyclonal anti-IFITM2 Cell signaling Cat# 13530S Rabbit polyclonal anti-IFITM3 Cell signaling Cat# 59212S V5-Tag (E9H8O) Mouse mAb Cell signaling Cat# 80076 SARS-CoV-2 (2019-nCoV) Nucleocapsid Antibody SinoBiological Cat# 40588-MM124 Rabbit polyclonal anti-ACE2 Abcam Cat# ab15348 Mouse monoclonal anti-flag Sigma-Aldrich Cat# F1804 Fixable Viability Dye eFluor 780 Thermo Fisher Cat# 65-0865-14 Rabbit monoclonal Alexa Fluor 647 anti-CD46 Abcam Cat# 275208 Rat Monoclonal PerCP-Cy5.5 anti-CD4 Biolegend Cat# 317428 Bacterial and virus strains VSVDG(GFP)*VSV-G Klaus Conzelmann N/A hCoV-229E ATCC Cat# VR-740TM hCoV-NL63 Lia van der Hoek N/A hCoV-OC43 ATCC Cat# CR-1558TM Renilla hCoV-229E Volker Thiel N/A BetaCoV/Netherlands/01/NL/2020 European Virus Archive Cat# 010V 03903 VSVDG(GFP)*VSV-G Klaus Conzelmann N/A BetaCoV/Netherlands/01/NL/2020 European Virus Archive Cat# 010V 03903 BetaCoV/B.1.617.2/Delta Florian Schmidt (University of Bonn) N/A BetaCoV/Munich/BavPat1/2020 GISAID; Wölfel et al., 202047 EPI_ISL_406862 SARS-CoV (rSCV, Frankfurt-1 virus isolate) Pfefferle et al., 200929 Genbank accession no. AY310120 rMERS-CoV, MERS-CoV/EMC2012 Muth et al., 201748 Genbank accession no. NC_019843.3 Chemicals, peptides, and recombinant proteins L-Glutamine Pan Biotech Cat# P04-80100 DAPI Sigma-Aldrich Cat# D9542-1MG; CAS: 28718-90-3 Dulbecco’s Modified Eagle Medium (DMEM) Gibco Cat# 41965039 Dulbecco’s Phosphate Buffered Saline (PBS) Thermo Fisher Cat# 14190094 Minimum Essential Medium Eagle (MEM) Sigma-Aldrich Cat# M4655 Opti-MEM Gibco Cat# M3942 Roswell Park Memorial Institute (RPMI) Gibco Cat# 22400089 Penicillin-Streptomycin Thermo Fisher Cat# 15140122 non-essential amino acids Sigma-Aldrich Cat# M7145 (Continued on next page) iScience 26, 106395, April 21, 2023 17 .. Sodium pyruvate Sigma-Aldrich Cat# S8636 FCS Gibco Cat# 10270106 Trypsin EDTA Gibco Cat# T3924 Versene Thermo Fisher Cat# 15040066 TMB peroxidase substrate (Sure blue) Medac Cat# 52-00-04 BD Cytofix/Cytoperm Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Lipofectamine RNAiMAX ThermoFisher Cat# 13778075 SuperScript III Platinum One-Step qRT-PCR Kit Invitrogen Cat# 11732088 Renilla Luciferase Assay kit Promega Cat# E2810 Quick RNA Miniprep Kit ZYMO research Cat# R1055 QIAamp Viral RNA Kits QIAGEN Cat# 52906 Luciferase Assay Kit Promega Cat# E1500 Mowiol 4-88 ROTH Cat# 0713.1 Blocker Casein in PBS Thermo Fisher Cat# 37528 TaqMan Fast Virus 1-Step Master Mix ThermoFisher Cat# 4444436 Tween 20 Sigma-Aldrich Cat# P7949500ML Triton X-100 Sigma-Aldrich Cat#T9284 100ML GAPD (GAPDH) Endogenous Control ThermoFisher Cat# 4352338E b-Mercaptoethanol Sigma-Aldrich Cat# 444203 Immobilon-FL PVDF membranes Merck Millipore Cat# IPVH00010 Paraformaldehyde Merck, Darmstadt Cat# 104005 TransIT-LT1 Mirus Cat# MIR2300 NuPAGE 4G12% Bis-Tris Gels ThermoFisher Cat# NP0321BOX Synthetic SARS-CoV-2-RNA Twist Bioscience Cat# 102019 RNAiMAX ThermoFisher Cat# LMRNA015 Recombinant IFN-a2 pbl assay science Cat# 11101-1 Recombinant IFN-b R&D systems Cat# 8499-IF-010 Recombinant IFN-g Sigma-Aldrich Cat# I3265 Recombinant IFN- l3 CHO Cat# 5259-IL-025 Cytochalasin D ThermoFisher Cat# PHZ1063 Agarose Sigma-Aldrich Cat# A9539 Experimental models: Cell lines Human: Calu-3 ATCC or M. Frick Cat# HTB-55 or N/A Human: Caco2 Holger Barth Human: Huh7.5 Ralf Bartenschlager Human: Huh7 Anna-Laura Kretz LLCMK2 by Lia van der Hoek
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